C y T O F
Mass Cytometry
CyTOF (Mass Cytometry)
The highest-resolution profiling of cell phenotype and function available
Biological Heterogeneity
Complexity of biological systems and analysis of high-dimensional proteomic panels
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Work Flow For CyTOF
Immune Profiling Assay
Human PBMC / Using 30 Biomarkers
t-SNE analysis [Immune cell subsets]
t-SNE gating analysis of immune cell subsets. Immune cell subsets were identified and manually gated in the t-SNE space according to the signal strength of the phenotypic marker.
t-SNE analysis [T lymphocyte subsets]
t-SNE gating analysis of T lymphocyte subsets. T lymphocyte subsets were identified and manually gated in the t-SNE space according to the signal strength of the phenotypic marker.
t-SNE analysis
Expression of biomarker displayed on t-SNE plot.
SPADE analysis
SPADE presents a spanning tree analysis of density normalization events. Through a combination of downsampling, clustering and minimal spanning tree, it provides intuitive visualization of high-dimensional single cell data to support data-driven cellular heterogeneity analysis.